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I am glad it is available now and thanks for the great work. Let’s not forget our programmers and the servers that provide such easy access to users. We appreciate your work and effort., VP1235, uncharacterized ORF, ORF1180 and VP1285 (*orf*) genes were identified in *Y. pseudotuberculosis* and *Y. enterocolitica*. None of these genes have been previously described to be involved in the negative regulation of iron uptake. Additionally, a recent study has identified these ORFs as putative virulence factors. [@pone.0102254-Pu1] In fact, Pu and collaborators, using a targeted inhibition approach, demonstrated that these ORFs are required for growth of the bacteria in human intestinal epithelial cells. Our data support the importance of *ybtS, ybtU* and *vp1235* genes in the regulation of iron uptake as the results of the deletion of these genes will cause a general increase in the iron content of the cells. Accordingly, we propose the term iron-potentiated gene (IPG) to describe the role of genes in the regulation of iron uptake.
In summary, we have analyzed in detail the influence of several members of the sRNA RsaA on biofilm formation and pathogenesis *in vivo* using an animal model of oral infection, *G. mellonella.* Our data show that all of the sRNAs are involved in biofilm formation, although there was little or no effect on virulence of *Y. pseudotuberculosis* YPIII in *G. mellonella.* While the sRNAs did not interfere with virulence, the qRT-PCR and *in vitro* assays for biofilm formation show that these sRNAs regulate the virulence phase. Furthermore, the role of RsaA and related sRNAs in the regulation of iron uptake is a new finding. The roles of these sRNAs in regulating the iron homeostasis *in vivo* and in *in vitro* iron uptake and biofilm formation are
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