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Purified human peripheral blood lymphocytes were used to study the effect of the presence of growth inhibition on the cytotoxicity of lymphocytes. Radioisotope labeled target cells were pretreated in serum-free media to remove inhibitory effects on growth before use as targets. In mixed lymphocyte populations the presence of inhibitory factors was found to result in a consistently higher level of cytotoxic activity. On the other hand, in primed lymphocyte cultures, the presence of growth inhibition resulted in the loss of cytotoxic activity. It was also shown that the cytotoxic activity of cytotoxic T lymphocytes (CTL) requires no other cell function than their ability to lyse sensitized target cells. When these requirements were imposed on the mixed lymphocyte population, no effect of growth inhibition could be shown. A hypothesis is formulated to explain these results.Novel lipidated protein delivery system for vaccine immunisation.
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